Recombinant Human MKK6 Protein (207 Ser/Asp, 211 Thr/Asp, His & GST Tag)(Active)
Size: 50μg
Catalogue Number: PKSH030414-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: MKK6
Target Symptom: MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3; SAPKK3; MAP2K6
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion Tag: N-His & GST
UNIProt ID: P52564
Accession: P52564-1
Background: Dual specificity mitogen-activated protein kinase kinase 6; also known as MAP kinase kinase 6; MAPKK 6; MAPK / ERK kinase 6; SAPKK3; MAP2K6 and MKK6; is a protein which belongs to the protein kinase superfamily; STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors; cytokines; ultraviolet light; and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle; heart; and in lesser extent in liver or pancreas.
Activity: The specific activity was determined to be 1250 nmol/min/mg using inactive MAPK14 as substrate.
Sequence: Met 1-Asp 334; Ser 207/Asp; Thr 211/Asp
Purity: > 88 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 65.3 kDa
Observed MW: 60 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only