Recombinant Human NAD Kinase/NADK Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032782-10, PKSH032782-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: NAD Kinase; NADK
Target Synonym: NAD Kinase; Poly(P)/ATP NAD Kinase; NADK
UNIProt ID: O95544
Research Areas: Signal Transduction; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: O95544
Background: NAD Kinase (NADK) is an enzyme that belongs to the NAD Kinase family. It is a widely expressed enzyme, but it is not detected in skeletal muscle. NADK converts Nicotinamide Adenine Dinucleotide (NAD+) into NADP+, through phosphorylating the NAD+ coenzyme. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined.
Sequence: Ser64-Gly446
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 44.4 kDa
Observed MW: 49 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only