Recombinant Human NCK1 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032339-10, PKSH032339-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: NCK1
Target Synonym: Cytoplasmic Protein NCK1; NCK adaptor Protein 1; Nck-1; SH2/SH3 Adaptor Protein NCK-Alpha; NCK1; NCK
UNIProt ID: P16333
Research Areas: Signal Transduction; Cancer; epigenetics and nuclear signal;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: P16333
Background: Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.
Sequence: Met 1-Ser377
Purity: > 80 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 5% Mannitol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 45.0 kDa
Observed MW: 50 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only