Recombinant Human NKG2DL/ULBP-1 Protein (Fc Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032815-10, PKSH032815-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: NKG2DL; ULBP-1
Target Synonym: NKG2D ligand 1; NKG2DL1; ALCAN-beta; Retinoic acid early transcript 1I; UL16-binding protein 1; ULBP1
UNIProt ID: Q9BZM6
Research Areas: immunology
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-Fc
Accession: Q9BZM6
Background: NKG2D ligand 1; also called ULBP1; is a member of UL16-binding protein (ULBP) family which has also been termed the retinoic acid early transcript 1 (RAET1) family. Unlike the classical MHC class I molecules and the MIC molecules possess α1; α2 and α3 domains; ULBP/RAET1 family members lack α3 domain. ULBP1 is recognized by the activating receptor NKG2D on the surface of cytotoxic natural killer (NK) and T cells; and then promotes the lysis of cells expressing ULBP1 which is important for the immune surveillance. ULBP1 and several other family members; ULBP2 and ULBP5; own the ability to bind the human cytomegalovirus (CMV) UL16 glycoprotein. The human CMV glycoprotein UL16 binds to intracellular ULBP1 and so inhibits its expression at the cell surface; which reduces the susceptibility of the virus-infected cell to cytotoxic destruction by NK cells. The expression of ULBP1 has been found on some tumor cells and is implicated in tumor surveillance.
Sequence: Gly26-Pro215
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.4 kDa
Observed MW: 58-70 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only