Recombinant Human NSE/ENO2 Protein
Sizes: 10ug, 50ug
Catalogue Number: RP02827-10, RP02827-50
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human NSE/ENO2 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Leu434) of human NSE/ENO2 (Accession #NP_001966.1) fused with a 6×His tag at the N-terminus.
Alternate Names: Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE; ENO2
SWISS: P09104
GeneID: 2026
Species: Human
Purity: ≥ 80 % as determined by SDS-PAGE.
Storage: Store at -20℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Gamma-enolase, also known as Enolase 2, belongs to the enolase family. The alpha/alpha homodimer of ENO2 is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jakob disease. ENO2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds to cultured neocortical neurons and promotes cell survival in a calcium-dependent manner.
Source: E. coli
Tag: N-His
Formulation: Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, 100mM KCl, 5mM MgSO4, pH 7.5.
Antigen Sequence: MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHNFRNPSVL
Endotoxin: < 1 EU/μg of the protein by LAL method.
Research Use Only