Recombinant Human Otolin-1 (C-6His)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033958-10, PKSH033958-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Otolin-1
Target Symptom: OTOL1; otolin 1; Otolin-1; C1qTNF15
Research Area: Signal Transduction;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-6His
UNIProt ID: A6NHN0
Accession: A6NHN0
Background: Otolin (OTOL1), also known as C1qTNF15, is an approximately 65 kDa protein found in the otoconial membrane lining the cochlea and vestibular labyrinth of the inner ear. Collagen-like protein specifically expressed in the inner ear, which provides an organic scaffold for otoconia, a calcium carbonate structure in the saccule and utricle of the ear. It associates into multimers and disulfide-linked oligomers and also associates with other otoconial proteins including and Otoconin-90 (also known as PLA2G2A, PLA2L, and phospholipase A2 homolog) and Cerebellin-1. It is extensively glycosylated and has multiple hydroxylated proline residues in the collagenous regions.
Sequence: Lys24-Pro477
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.7 kDa
Observed MW: 84-94 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only