Recombinant Human P21 protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100242-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: P21
Target Synonym: CAP20; CDK-interacting protein 1; CDKI; CDKN1; CDN1A; CIP1; CDKN1A; Cyclin Dependent Kinase Inhibitor 1A; DNA Synthesis Inhibitor; MDA-6; MDA6; Melanoma differentiation-associated protein 6; P21cip1; P21waf; PIC1; SDI1; SLC12A9; WAF1
UNIProt ID: P38936
Research Areas: Signal Transduction; Cancer; Epigenetics and Nuclear Signaling; Cell Biology
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: P38936
Background: May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
Sequence: Gly 40-Pro 164
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 13.64 kDa
Observed MW: 18 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only