Recombinant Human PFKFB3 (N-6His)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033971-10, PKSH033971-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PFKFB3
Target Symptom: IPFK2; PFK2; iPFK-2; PFK/FBPase 3
Research Area: Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism;
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
UNIProt ID: Q16875
Accession: Q16875
Background: Fructose-2, 6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, involved in both the synthesis and degradation of fructose-2, 6-bisphosphate, a regulatory molecule that controls the activity of the enzymes phosphofructokinase 1 (PFK-1) and fructose 1, 6-bisphosphatase (FBPase-1) to regulate glycolysis and gluconeogenesis. Highly phosphorylated PFKFB3 protein was found in human tumor cells, vascular endothelial cells, and smooth muscle cells. Because of its proto-oncogenic character, the PFK-2/FBPase-2 of the PFKFB3 gene is assumed to play a critical role in tumorigenesis. PFKFB3 also plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions, which makes it a potential target for cancer therapy.
Sequence: Met1-His520
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 20% Glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 61.8 kDa
Observed MW: 57-62 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only