Recombinant Human PKC epsilon/PKCE Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032964-10, PKSH032964-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PKC epsilon; PKCE
Target Synonym: Protein Kinase C Epsilon Type; nPKC-Epsilon; PRKCE; PKCE
UNIProt ID: Q02156
Research Areas: Signal Transduction; Cardiovascular; Cancer;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: Q02156
Background: Protein Kinase C Epsilon type is a member of the serine- and threonine-specific protein kinase family that can be activated by calcium and the second messenger diacylglycerol. Protein Kinase C Epsilon contains these domains: one AGC-kinase C-terminal domain; one C2 domain; one protein kinase domain and two phorbol-ester/DAG-type zinc fingers. Protein Kinase C Epsilon phosphorylate a variety of protein targets and has been identified to participate in diverse cellular signaling pathways. It has many different cellular functions; such as neuron channel activation; apoptosis; cardioprotection from ischemia; heat shock response; as well as insulin exocytosis. Protein Kinase C Epsilon also serves as the receptor for phorbol esters; a class of tumor promoters.
Sequence: Gln580-Pro737
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 19.6 kDa
Observed MW: 20 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only