Recombinant Human PPM1A protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100431-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: PPM1A
Target Synonym: Protein Phosphatase 1A; Protein Phosphatase 2C Isoform Alpha; PP2C-Alpha; Protein Phosphatase IA; PPM1A; PPPM1A; PP2C-ALPHA; PP2CA; PP2Calpha
UNIProt ID: P35813
Research Areas: signal Transduction;
Epigenetics and Nuclear
signaling
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His
Accession: P35813
Background: Protein Phosphatase 1A (PPM1A) is a member of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1A has a broad specificity. PPM1A negatively regulates the activities of MAP kinases and MAP kinase kinases. Also; it negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3; resulting in their dissociation from SMAD4; nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. In addition; PPM1A can dephosphorylate cyclin-dependent kinases; and thus may be involved in cell cycle control.
Sequence: Gly2-Trp382
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 41.8 kDa
Observed MW: 45 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only