Recombinant Human PPM1G/PP2C-gamma Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032970-10, PKSH032970-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PPM1G; PP2C-gamma
Target Synonym: Protein Phosphatase 1G; Protein Phosphatase 1C; Protein Phosphatase 2C Isoform Gamma; PP2C-Gamma; Protein Phosphatase Magnesium-Dependent 1 Gamma; PPM1G; PP2CG; PP2CGAMMA
UNIProt ID: O15355
Research Areas: Signal Transduction; Cell biology; epigenetics and nuclear signal;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: O15355
Background: Protein Phosphatase 1G (PP1MG) is a cytoplasmic protein that belongs to the PP2C family. PPM1G is widely expressed; it is abundant in testis; skeletal muscle; and heart. PPM1G is a negative regulator of cell stress response pathways. PPM1G is responsible for the dephosphorylation of Pre-mRNA splicing factors; an important factor for the formation of functional spliceosome. PPM1G also plays a role in regulating cell cycle progression.
Sequence: Met317-Asp546
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 1mM DTT, 1mM EDTA, 2mM β-ME, 20% Glycerol, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 26.2 kDa
Observed MW: 30 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only