Recombinant Human PPP1R14A Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032967-10, PKSH032967-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PPP1R14A
Target Synonym: Protein Phosphatase 1 Regulatory Subunit 14A; 17 kDa PKC-Potentiated Inhibitory Protein of PP1; Protein Kinase C-Potentiated Inhibitor Protein of 17 kDa; CPI-17; PPP1R14A; CPI17; PPP1INL
UNIProt ID: Q96A00
Research Areas: Signal Transduction;
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
Accession: Q96A00
Background: Protein Phosphatase 1 Regulatory Subunit 14A (PPP1R14A) belongs to the PP1 inhibitor family. PPP1R14A is mapped to chromosome 19q13.13-q13.2. PPP1R14A binds directly to protein kinase C and casein kinase I. Meantime; PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. PPP1R14A is the inhibitor of PPP1CA. When phosphorylated; PPP1R14A has over 1000-fold higher inhibitory activity; creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. In addition; inhibition of PPP1R14A also enhances contraction of smooth muscle in the absence of increment of intracellular Ca2+ concentration.
Sequence: Met 1-Pro147
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 0.2mM EDTA, 1mM DTT, 10% Glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 17.8 kDa
Observed MW: 22 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only