Recombinant Human PRLR/Prolactin Receptor Protein (His Tag) - PKSH031713

Recombinant Human PRLR/Prolactin Receptor Protein (His Tag)

Size: 100μg

Catalogue Number: PKSH031713-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: PRLR, Prolactin Receptor

Target Synonym: HPRL, hPRLrI, MFAB, PRLR

Research Areas: Signal Transduction, Cancer, metabolism,

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-His

Accession: NP_000940.1

Background: Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type &acirc, &hellip, cytokine receptor superfamily, and contains two fibronectin type-&acirc, &hellip, &cent, domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules invloved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.

Sequence: Met 1-Asp 234

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 26 kDa

Observed MW: 36-38 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only