Recombinant Human PSA/KLK3 Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSH031436-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: PSA; KLK3
Target Symptom: APS; Hk3; KLK2A1; PSA; Prostate-Specific Antigen; PSA; Gamma-Seminoprotein; Seminin; Kallikrein-3; P-30 Antigen; Semenogelase;
Research Area: Signal Transduction; Tags & Cell Markers; Neuroscience; Cancer; immunology;
Target Species: Human
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-His
UNIProt ID: P07288
Accession: P07288
Background: KLK3(Kallikrein 3); also known as Prostate Specific Antigen (PSA); kallikrein-related peptidase 3; Gamma-seminoprotein; is a secreted protein of the glandular kallikrein subfamily of serine proteases.KLK3 contains one peptidase S1 domain.KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Activity: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide(Suc-RPY-pNA). The specific activity is > 100 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Sequence: Met 1-Pro 261
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 28.3 kDa
Observed MW: 35 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only