Recombinant Human RHEB Protein (His Tag)
Size: 100μg
Catalogue Numbers: PKSH031137-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: RHEB
Target Synonym: GTP-Binding Protein Rheb; Ras Homolog Enriched in Brain; RHEB; RHEB2
UNIProt ID: Q15382
Research Areas: Signal Transduction; Cancer; metabolism;
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Fusion Tag: N-His
Accession: Q15382
Background: RHEB is a recently discovered member of the Ras superfamily that may be involved in neural plasticity. This function is novel and not typically associated with the Ras proteins. RHEB gene is a member of the small GTPase superfamily and encodes a lipid-anchored; cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is vital in regulation of growth and cell cycle progression due to its role in the insulin / TOR / S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form; and farnesylation of RHEB is required for this activity. Three pseudogenes have been mapped; two on chromosome 10 and one on chromosome 22.
Sequence: Met 1-Cys181
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 22.4 kDa
Observed MW: 21 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only