Recombinant Human RSPO3 Protein (Fc & His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033378-10, PKSH033378-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: RSPO3
Target Synonym: R-spondin-3; RSPO3; Protein with TSP type-1 repeat; Roof plate-specific spondin-3; Thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1
UNIProt ID: Q9BXY4
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-Fc-6His
Accession: Q9BXY4-1
Background: R-spondin-3 (RSPO3); also known as Protein with TSP type-1 repeat; Roof plate-specific spondin-3; Thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2 and CRISTIN1; is a member of the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein and widely expressed in many tissues. RSPO3 contains two Furin-like repeats which have been found in a variety of eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases; and one TSP type-1 domain; RSPO3 founctions as a activator of the beta-catenin signaling cascade; leading to TCF-dependent gene activation. Otherwise; RSPO3 may negatively regulate the TGF-beta pathway.
Sequence: Gln22-His272
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl; pH7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 56.3 kDa
Observed MW: 61 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only