Recombinant Human SCF/c-Kit Ligand Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033081-10, PKSH033081-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: SCF; c-Kit Ligand
Target Symptom: Kit Ligand; Mast Cell Growth Factor; MGF; Stem Cell Factor; SCF; c-Kit ligand; KITLG; MGF; SCF
Research Area: Developmental Biology; immunology; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture
Fusion Tag: C-6His
UNIProt ID: P21583
Accession: P21583
Background: Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid; erythroid; and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
Activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 2-10 ng/ml.
Sequence: Glu26-His214
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl; pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 22.0 kDa
Observed MW: 30-50 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only