Recombinant Human Semaphorin 3A/SEMA3A Protein (His & Flag Tag) - PKSH033535

Recombinant Human Semaphorin 3A/SEMA3A Protein (His & Flag Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033535-10, PKSH033535-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Semaphorin 3A; SEMA3A

Target Symptom: Semaphorin III; SEMA3A; SEMAD; Hsema-I; SEMA1; SemD; Semad; coll-1; Hsema-III; Sema III

Research Area: Cardiovascular; Neuroscience

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: N-His-Flag

UNIProt ID: Q14563

Accession: Q14563

Background: emaphorin-3A is a secreted protein which belongs to the semaphorin family. Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 500 aa Sema domain.This protein can function as either a chemorepulsive agent; inhibiting axonal outgrowth; or as a chemoattractive agent; stimulating the growth of apical dendrites. In both cases; the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines.

Sequence: Lys26-Phe546

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 61.3 kDa

Observed MW: 70-75 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only