Recombinant Human SIGLEC9/CD329 Protein (His Tag)(Active)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033787-10, PKSH033787-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: SIGLEC9; CD329
Target Symptom: Sialic acid-binding Ig-like lectin 9; Siglec-9; CDw329; Protein FOAP-9; SIGLEC9
Research Area: Tags & Cell Markers; Cardiovascular; immunology
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture; ELISA
Fusion Tag: C-His
UNIProt ID: Q9Y336
Accession: Q9Y336
Background: Sialic acid-binding Ig-like lectin 9(Siglec 9) is expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils); and found in liver; fetal liver; bone marrow; placenta; spleen and in lower levels in skeletal muscle; fetal brain and so on. It is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It also binds to alpha-2; 3- or alpha-2; 6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Activity: Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Approximately 93% will adhere after 5-10 mins incubation at 37 °C.
Sequence: Gln18-Gly348(Lys100Glu; Ala315Glu)
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS; 2mMEDTA; ph7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 36.9 kDa
Observed MW: 55-90 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only