Recombinant Human SLPI Protein (aa 1-132, His Tag)(Active)
Size: 100μg
Catalogue Number: PKSH031665-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: SLPI
Target Symptom: ALK1; ALP; BLPI; HUSI; HUSI-I; MPI; WAP4; WFDC4
Research Area: Cell biology; Cardiovascular; immunology
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion Tag: C-His
UNIProt ID: P03973
Accession: P03973
Background: Secretory leukoprotease inhibitor (SLPI); also called antileukoprotease (ALP); is a 12-kDa; nonglycosylated serine protease inhibitor present in mucous secretions. It is thought to play a role in protecting the mucosae from injury associated with inflammation. SLPI is locally produced by serous cells; including bronchial submucosal glands. Elafin and SLPI are members of larger families of proteins secreted predominantly at mucosal sites; and have been shown to be modulated in multiple pathological conditions. Elafin and SLPI are structurally related in that both have a fold with a four-disulfide core or whey acidic protein (WAP) domain responsible for inhibiting proteases. SLPI is a prominent innate immune protein of the respiratory tract; possessing serine protease inhibitor activity; antibacterial activity; and anti-inflammatory/immunomodulatory activity.
Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2 (Catalog # ES002). The IC50 value is < 1 Nm.
Sequence: Met 1-Ala132
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 13.1 kDa
Observed MW: 15 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only