Recombinant Human SMAD1 Protein (GST Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033065-10, PKSH033065-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: SMAD1
Target Synonym: Mothers Against Decapentaplegic Homolog 1; MAD Homolog 1; Mothers Against DPP Homolog 1; JV4-1; Mad-Related Protein 1; SMAD Family Member 1; Transforming Growth Factor-Beta-Signaling Protein 1; BSP-1; SMAD1; BSP1; MADH1; SMAD 1; Smad1; hSMAD1; MADR1
UNIProt ID: Q15797
Research Areas: Signal Transduction; Cancer; epigenetics and nuclear signal; metabolism; Stem cells
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-GST
Accession: Q15797
Background: SMAD Family Member 1 (SMAD1) is a member of the dwarfin/SMAD family. SMAD1 has the highest expression in the heart and skeletal muscle, containing one MAD homology 1 domain and one MAD homology 2 domain, As a transcriptional modulator SMAD 1 is activated by bone morphogenetic proteins type 1 receptor kinase. Defects in SMAD1 may cause primary pulmonary hypertension (PPH1), characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure and death.
Sequence: Met 1-Ser465
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0 .
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 78.7 kDa
Observed MW: 28&89 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only