Recombinant Human SMYD3/ZMYND1 Protein (GST Tag)
Size: 50μg
Catalogue Number: PKSH031202-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: SMYD3, ZMYND1
Target Synonym: bA74P14.1, KMT3E, ZMYND1, ZNFN3A1
Research Areas: Cancer, epigenetics and nuclear signal
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Fusion Tag: N-GST
Accession: NP_073580.1
Background: SET and MYND domain-containing protein 3, also known as Zinc finger MYND domain-containing protein 1, SMYD3, and ZMYND, is a member of the histone-lysine methyltransferase family. SMYD3 contains oneMYND-type zinc finger and oneSET domain. SMYD3 is a histone H3 lysine-4-specific methyltransferase. It is expressed in skeletal muscles and testis. It is overexpressed in a majority of colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC). SMYD3 plays an important role in transcriptional regulation in human carcinogenesis. It activates the transcription of a set of downstream genes. Of these downstream genes, there are several oncogenes and genes associated with cell adhesion (including those of N-Myc, CrkL, Wnt10b, L-selectin, CD31 and galectin-4), which have been shown to have effects on cell viability, adhesion, migration and metastasis. Increased SMYD3 expression is essential for the proliferation of breast cancer cells. SMYD3 may be a promising new target of therapeutic intervention for the treatment of cancers or other pathological processes associated with cell adhesion and migration.
Sequence: Lys 35-Ser 369
Purity: > 88 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.5mM DTT, 0.5mM GSH, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 656 kDa
Observed MW: 58 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only