Recombinant Human Somatotropin/GH-N/GH1 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP00033-10, RP00033-20, RP00033-50, RP00033-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Human Somatotropin/GH-N/GH1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Phe27-Phe217) of human GH (Accession #NP_000506.2) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.
Alternate Names: GH1, GH, GH-N, GHB5, GHN, IGHD1B, hGH-N, somatotropin, GH, GH-N, GHB5, GHN, IGHD1B, hGH-N
SWISS: P01241
GeneID: 2688
Species: Human
Purity: ≥ 90 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones - GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin and leptin. Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types. GH stimulates the liver and other tissues to produce IGF-1, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-1. GH, directly or indirectly via IGF-1, can act on B cells, T cells, NK cells, macrophages and neutrophils to exert immunomodulatory activities. In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake and protein synthesis.
Bio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GH1 at 2 μg/mL (100 μL/well) can bind Human GHR with a linear range of 0.1-19.4 ng/mL.
Source: E. coli
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation.
Antigen Sequence: FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only