Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)(Active)
Sizes: 20μg, 100μg
Catalogue Numbers: PKSH031645-20, PKSH031645-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Sonic Hedgehog; SHH
Target Symptom: Sonic Hedgehog Protein; SHH; HHG-1; SHH
Research Area: Cancer; Developmental Biology; epigenetics and nuclear signal; metabolism; Stem cells
Target Species: Human
Expression Host: HEK293 Cells
Application: Cell culture
Fusion Tag: C-His
UNIProt ID: Q15465
Accession: Q15465
Background: Sonic HedgeHog; also known as sonic hedgehog protein; belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine; liver; lung; and kidney. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube; the anterior-posterior limb axis; and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites; and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor; which functions in association with smoothened; to activate the transcription of target genes. In the absence of sonic HedgeHog; patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor; the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5; triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3.
Activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-10 μg/mL.
Sequence: Met 1-Gly 197
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 21 kDa
Observed MW: 24 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only