Recombinant Human SPHK1/Sphingosine Kinase 1 Protein (Active)
Size: 50μg
Catalogue Number: PKSH030315-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: SPHK1; Sphingosine Kinase 1
Target Symptom: SPHK; SPHK1; SPK
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion Tag: None
UNIProt ID: Q9NYA1
Accession: Q9NYA1-1
Background: SPHK1; also known as sphingosine Kinase 1; catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation; survival; migration; cytoskeletal organization; and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory; antiapoptotic; and immune processes.
Activity: The specific activity was determined to be 1028 nmol/min/mg using Sphingosine Kinase Substrate as substrate.
Sequence: Met 1-Leu 384
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 42.7 kDa
Observed MW: 46 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only