Recombinant Human STK40 Protein (His & GST Tag)
Size: 50μg
Catalogue Number: PKSH030358-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: STK40
Target Synonym: SgK495, SHIK
Research Areas: Signal Transduction, Cell biology, epigenetics and nuclear signal,
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Fusion Tag: N-His & GST
Accession: NP_114406
Background: STK40 localized to both the cytoplasm and the nucleus. It is ubiquitously expressed. Mechanistically, Stk40 interacts with Rcn2, which also activates Erk1/2 to induce ExEn specification in mouse ESCs. Stk40 is able to activate the Erk/MAPK pathway and induce extraembryonic-endoderm (ExEn) differentiation in mouse ESCs. Interestingly, cells overexpressing Stk40 exclusively contribute to the ExEn layer of chimeric embryos when injected into host blastocysts. In contrast, deletion of Stk40 in ESCs markedly reduces ExEn differentiation in vitro. STK40 has a central serine/threonine protein kinase domain and is homologous to TRB-3, a protein that regulates activation of MAP kinases and inhibits NF&kappa, B-mediated gene transcription. Similarly, overexpression of STK40 inhibits NF&kappa, B activation triggered by TNF and also inhibits p53-mediated transcription. There are four named isoforms of STK40 that are produced as a result of alternative splicing.
Sequence: Met 1-Lys 435
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, 3mM DTT, 0.5M Urea, 0.5mM GSH, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 76.8 kDa
Observed MW: 85 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only