Recombinant Human STXBP1/UNC18A Protein (His & GST Tag)
Size: 100μg
Catalogue Number: PKSH031010-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: STXBP1, UNC18A
Target Synonym: MUNC18-1, NSEC1, P67, RBSEC1, UNC18
UNIProt ID: P61764
Target Species: Human
Expression Host: Baculovirus-Insect Cells
Fusion Tag: N-His & GST
Accession: P61764-1
Background: Syntaxin-binding protein 1, also known as N-Sec1, Protein unc-18 homolog 1, MUNC18-1 and STXBP1, is a peripheral membrane protein which belongs to theSTXBP / unc-18 / SEC1 family. STXBP1 is an evolutionally conserved neuronal Sec1/Munc-18 (SM) protein that is essential in synaptic vesicle release in several species. It may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. STXBP1 is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. It can interact with syntaxins 1, 2, and 3 but not syntaxin 4. STXBP1 may also play a role in determining the specificity of intracellular fusion reactions. Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4). Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
Sequence: Met 1-Ser 594
Purity: > 85 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 95.4 kDa
Observed MW: 80 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only