Recombinant Human SUMF1 Protein (His Tag) - PKSH033084

Recombinant Human SUMF1 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033084-10, PKSH033084-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: SUMF1

Target Synonym: Sulfatase-Modifying Factor 1; C-Alpha-Formylglycine-Generating Enzyme 1; SUMF1; FGE

UNIProt ID: Q8NBK3

Research Areas: Signal Transduction; Stem cells

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

Accession: Q8NBK3

Background: Human Sulfatase Modifying Factor 1 (SUMF1) is a 42kDa protein. SUMF1 is a Ca2+-binging member of the sulfatase-modifying factor family. SUMF1 is a soluble ER lumenal glycoprotein, it converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, SUMF1 can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF2. Three splice isoforms are known.

Sequence: Ser34-Asp374

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 2mM CaCl2, 10% Glycerol, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 38.3 kDa

Observed MW: 38-42 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only