Recombinant Human TNFSF5/CD40 ligand/CD154 Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01733-10, RP01733-20, RP01733-50, RP01733-100
Citations, Manuals and MSDS Available upon request.
Description: Active Recombinant Human CD40-L/CD40L/TNFSF5/TRAP/CD40LG Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly116-Leu261) of human CD40-L/CD40L/TNFSF5/TRAP/CD40LG(Accession #NP_000065.1) fused with and a 6×His tag at the N-terminus.
Alternate Names: IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40LG
SWISS: P29965
GeneID: 959
Species: Human
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.
Bio-Activity: Measured by its binding ability in a functional ELISA.Immobilized Human CD40L (Catalog: RP01733) at 5 μg/mL (100 μL/well) can bind Human CD40 (Catalog: RP01218) with a linear range of 0.005-0.5 μg/mL.
Source: HEK293 cells
Tag: N-6His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: GDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only