Recombinant Human TRIM5/RNF88 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033149-10, PKSH033149-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: TRIM5; RNF88
Target Synonym: Tripartite motif-containing protein 5; RING finger protein 88; TRIM5; RNF88
UNIProt ID: Q9C035
Research Areas: Cell biology; epigenetics and nuclear signal; immunology
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-6His
Accession: Q9C035
Background: Tripartite motif-containing Motif 5 is a protein that in humans is encoded by the TRIM5 gene.It is a 493 amino acids protein that belongs to the TRIM/RBCC family.It contains 1 B box-type zinc finger, 1 B30.2/SPRY domain and 1 RING-type zinc finger. TRIM5 present in the cytoplasm recognizes motifs within the capsid proteins and interferes with the uncoating process, therefore preventing successful reverse transcription and transport to the nucleus of the viral genome. The exact mechanism of action has not been shown conclusively, but capsid protein from restricted viruses is removed by proteasome-dependent degradation
Sequence: Met 1-Gln248
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 30.8 kDa
Observed MW: 33 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only