Recombinant Human Troponin C/TNNC1 Protein
Size: 100μg
Catalogue Number: PKSH031309-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Troponin C, TNNC1
Target Synonym: CMD1Z, CMH13, TN-C, TNC, TNNC
Research Areas: Signal Transduction, Cardiovascular,
Target Species: Human
Expression Host: E.coli
Fusion Tag: None
Accession: NP_003271.1
Background: Troponin I, also known as TNNC1, is part of the troponin complex. This complex contains 3 subunits: troponin I (TnI), troponin T (TnT) and troponin C (TnC). Troponin I is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.
Sequence: Met 1-Glu161
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 150 mM NaCl, 10 mM Na2HPO4, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 18.4 kDa
Observed MW: 20 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only