Recombinant Human UBE2M/UBC12 Protein
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033478-10, PKSH033478-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: UBE2M; UBC12
Target Symptom: NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M; UBC12; UBE2M;
Research Area: Cell biology; epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: None
UNIProt ID: P61081
Accession: P61081
Background: UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. This protein is linked with a ubiquitin-like protein; NEDD8; which can be conjugated to cellular proteins; such as Cdc53/culin. UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1; but not RBX2; suggests that the RBX1-UBE2M complex neddylates specific target proteins; such as CUL1; CUL2; CUL3 and CUL4. It involved in cell proliferation and is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Sequence: Met1-Lys183
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 50mM HEPES, pH 7.5, 2mM DTT, 150mM NaCl, 10% Glycerol.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 20.9 kDa
Observed MW: 25 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only