Recombinant Human UBE2S Protein (GST Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033189-10, PKSH033189-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: UBE2S
Target Symptom: Ubiquitin-Conjugating Enzyme E2 S; E2-EPF; Ubiquitin Carrier Protein S; Ubiquitin-Conjugating Enzyme E2-24 kDa; Ubiquitin-Conjugating Enzyme E2-EPF5; Ubiquitin-Protein Ligase S; UBE2S; E2EPF
Research Area: epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-GST
UNIProt ID: Q16763
Accession: Q16763
Background: Ubiquitin-Conjugating Enzyme E2 S (UBE2S) is a member of the Ubiquitin-Conjugating Enzyme family. UBE2S interacts with CDC20, FZR1/CDH1 and VHL. UBE2S can form a thiol ester linkage with Ubiquitin in an Ubiquitin Activating Enzyme-Dependent manner, a characteristic property of Ubiquitin Carrier Proteins. UBE2S acts as an essential factor of the Anaphase Promoting Complex/Cyclosome, a cell cycle-regulated Ubiquitin ligase that controls progression through mitosis. UBE2S is also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A.
Sequence: Met 1-Leu222
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 50.1 kDa
Observed MW: 50 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only