Recombinant Human UCHL3/UCH-L3 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033176-10, PKSH033176-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: UCHL3; UCH-L3
Target Symptom: Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3; UCH-L3; Ubiquitin Thioesterase L3; UCHL3
Research Area: Cell biology; epigenetics and nuclear signal
Target Species: Human
Expression Host: E.coli
Fusion Tag: C-6His
UNIProt ID: P15374
Accession: P15374
Background: Ubiquitin Carboxyl-Terminal Hydrolases (UCHs) are a family of cysteine hydrolases. They catalyze the hydrolysis of amides, thioesters and esters, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin. Up regulation of UCHL3 is associated with uterine cervical neoplasms. UCHL3 is implicated in age related cognitive disorders. UCHL3 also promotes adipogenesis and insulin signaling. In mice, UCHL3 knockout have been shown to be resistant to diet-induced obesity.
Sequence: Met 1-Ala230
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% Glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 27.3 kDa
Observed MW: 25 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only