Recombinant Human Urokinase/uPA protein (His tag)
Sizes: 20μg, 100μg
Catalogue Numbers: PDMH100158-20, PDMH100158-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Urokinase; uPA
Target Synonym: Urokinase-Type Plasminogen Activator; U-Plasminogen Activator; uPA; PLAU; ATF; BDPLT5; QPD; u-PA; UPA; URK
UNIProt ID: P00749
Research Areas: Cardiovascular; Neuroscience; Cancer; metabolism
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
Accession: P00749
Background: Recombinant Human Urokinase-Type Plasminogen Activator is a serine protease, which specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. Urokinase-Type Plasminogen Activator is a potent marker of invasion and metastasis in many human cancers associated with breast, colon, stomach, bladder, brain, ovary and endometrium. Human Urokinase-Type Plasminogen Activator is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide residues. The single chain molecule is processed into a disulfide-linked two-chain molecule. There exists two forms A chain, the long A chain contains an EGF-like domain that is responsible for binding of the uPA receptor. The B chain corresponds to the catalytic domain.
Sequence: Met1-Leu431
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 47.3 kDa
Observed MW: 45 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only