Recombinant Human Vaspin/SerpinA12 Protein (His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH033533-10, PKSH033533-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Vaspin; SerpinA12
Target Symptom: Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; SERPINA12
Research Area: Signal Transduction; Cell biology; Cardiovascular; Neuroscience; Cancer; metabolism;
Target Species: Human
Expression Host: HEK293 Cells
Fusion Tag: C-His
UNIProt ID: Q8IW75
Accession: Q8IW75
Background: Vaspin (Visceral Adipose-Specific SERPIN) is a newly described adipokine. Vaspin has three β-sheets; nine α-helices; and one central loop; the structure is part of the set of distinctive features that are descriptive of Serpin family members. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that Vaspin mRNA expression in visceral fat is positively correlated with BMI and percent of body fat. and could be associated with parameters of obesity; insulin resistance; and glucose metabolism. These findings suggest a potential clinical use for Vaspin in ameliorating certain aberrations seen in the obesity metabolic syndrome.
Sequence: Leu21-Lys414
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 46.5 kDa
Observed MW: 50-60 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only