Recombinant Human WDYHV1/NTAQ1 Protein (GST Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSH032966-10, PKSH032966-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: WDYHV1; NTAQ1
Target Synonym: Protein N-terminal glutamine amidohydrolase; WDYHV1; Protein NH2-terminal glutamine deamidase; N-terminal Gln amidase; Nt(Q)-amidase; C8orf32; NTAQ1
UNIProt ID: Q96HA8
Research Areas: Cell biology
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-GST
Accession: Q96HA8
Background: Human protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However, it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
Sequence: Met 1-Cys205
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of PBS, 100mM GSH, 1% TritonX-100, 15% Glycerol, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.8 kDa
Observed MW: 45-50 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only