Recombinant Human Zic3 protein (His tag)
Size: 100μg
Catalogue Numbers: PDEH100321-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Zic3
Target Synonym: Zinc finger protein ZIC 3; ZIC3; Zinc finger protein 203; Zinc finger protein of the cerebellum 3
UNIProt ID: O60481
Research Areas: Developmental Biology; Epigenetics and Nuclear signaling; Stem Cells
Target Species: Human
Expression Host: E.coli
Fusion Tag: N-His & C-His
Accession: O60481
Background: ZIC3 (zinc-finger protein of the cerebellum 3) is a 50 kDa (predicted) member of the GLI C2H2-type Zn-finger protein family. It is expressed in cerebellum, and apparently plays a key role in left-right body axis formation. Human ZIC3 is 467 amino acids (aa) in length. It contains N-terminal poly-Ala (aa 46-55) and poly-His (aa 87-97) segments, followed by five C2H2-type Zn-fingers (aa 253-410). Within the zinc finger region, there is an overlapping NES (aa 290-349) and two NLS's (aa 367-382 and 403-410). ZIC3 reportedly binds to GLI-3.
Sequence: Met 1-Val467
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 51.26 kDa
Observed MW: 52 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only