Recombinant IGF-I, Human
Catalogue Numbers: BK0084-10, BK0084-50
Sizes: 10μg, 50μg
Source: Escherichia coli
Molecular Weight: 7.7 kDa, observed by reducing SDS-PAGE.
Purity: > 95% by SDS-PAGE and HPLC analyses.
Biological Activity: ED50 < 5 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0× 10ˆ5 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against PBS.
AA Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O at 100 μg/ml.
Storage: Lyophilized recombinant human Insulin-like growth factor I (rhIGF-I) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIGF-I should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Insulin-like growth factor I (IGF-I) also known as Somatamedin C is a hormone similar in molecular structure to insulin. Human IGF-I has two isoforms (IGF-IA and IGF-IB) which is differentially expressed by various tissues. Mature human IGF-I respectively shares 94% and 96% aa sequence identity with mouse and rat IGF-I. Both IGF-I and IGF-II (another ligand of IGF) can signal through the IGF-I receptor (IGFIR), but IGF-II can alone bind the IGF-II receptor (IGFIIR/ Mannose-6-phosphate receptor). IGF-I plays an important role in childhood growth and continues to have anabolic effects in adults. Recombinant human Insulin-like growth factor I (rhIGF-I) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhIGF-I has a molecular mass of 7.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.