Recombinant IL-3, Mouse
Catalogue Numbers: BK0103-10, BK0103-50
Sizes: 10μg, 50μg
Source: Escherichia coli
Molecular Weight: 15.2 kDa, observed by non-reducing SDS-PAGE.
Purity: > 95% by SDS-PAGE analysis.
Biological Activity: ED50 < 0.05 ng/mL, measured by a cell proliferation assay using M-NFS-60 cells, corresponding to a specific activity of > 2 × 10ˆ7 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against PBS.
AA Sequence: MDTHRLTRT LNCSSIVKEI IGKLPEPELK TDDEGPSLRN KSFRRVNLSK FVESQGEVDP
EDRYVIKSNL QKLNCCLPTS ANDSALPGVF IRDLDDFRKK LRFYMVHLND LEt VLTSRPP
QPASGSVSPN RGt VEC
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O at 100 μg/mL.
Storage: Lyophilized recombinant mouse Interleukin-3 (rmIL-3) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-3 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific α chain and a common β chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family. Recombinant mouse Interleukin-3 (rmIL-3) produced in E.coli is a single non-glycosylated polypeptide chain containing of 135 amino acids. A fully biologically active molecule, rmIL-3 has a molecular mass of 15.2 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.