Recombinant MCP-2/CCL8, Human
Catalogue Numbers: BK0126-5, BK0126-25
Sizes: 5μg, 25μg
Source: Escherichia coli
Molecular Weight: 8.9 kDa, observed by reducing SDS-PAGE.
Purity: > 95% by SDS-PAGE analysis.
Biological Activity: ED50< 0.5 µg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL8, corresponding to a specific activity of > 2 × 10ˆ3 units/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized after extensive dialysis against PBS.
AA Sequence: QPD SVSIPITCCF NVINRKIPIQ RLESYTRITN IQCPKEAVIF KTKRGKEVCA DPKERWVRDS MKHLDQIFQN LKP
Endotoxin: < 0.2 EU/μg, determined by LAL method.
Reconstitution: Reconstituted in ddH2O at 100 μg/mL.
Storage: Lyophilized recombinant human MCP-2/CCL8(rhMCP-2) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMCP-2 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Description: MCP-2 is a member of the chemokines, a group of 70-80 residue proteins sharing substantial sequence similarity. Within the chemokines, MCP-2 belongs to the CC subfamily, and is a member of the Monocyte Chemoattractant Proteins (MCPs), which includes MCP-1, MCP-2, MCP-3, MCP-4, and MCP-5. MCP-2 shares 60% homology with MCP-1, and both proteins can undergo reversible dimerization. The main receptors of MCP-2 are G-protein coupled receptors CCR1 and CCR5. MCP-2 is a potential target in HIV-1 infected human glial cells as it may play a role in the modulation of viral spread in the brain. Recently, researchers found that mouse MCP-2 is expressed in the skin as a novel agonist of CCR8 and plays a role in eosinophilic inflammation.Recombinant human MCP-2/CCL8(rhMCP-2) produced in E.coli is a single non-glycosylated polypeptide chain containing 76 amino acids. A fully biologically active molecule, rhMCP-2 has a molecular mass of 8.9kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.