Recombinant MERS-CoV Nucleocapsid Protein
Sizes: 50μg, 500μg
Catalogue Numbers: PKSV030288-50, PKSV030288-500
Citations, Manuals and MSDS Available upon request.
Abbreviation: Nucleocapsid
Target Synonym: MERS-CoV Nucleocapsid Protein; MERS-CoV coronavirus NP Protein; MERS-CoV np Protein; MERS-CoV novel coronavirusNucleoprotein Protein
UNIProt ID: K0BVN3
Target Species: MERS
Expression Host: E.coli
Fusion Tag: N-6His
Accession: K0BVN3
Background: Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Sequence: Met1-Asp413
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl 500mM NaCl, 0.1% Chaps, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: Please contact us for more information.
Calculated MW: 48.8 kDa
Observed MW: 53 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only