Recombinant MERS-CoV Nucleoprotein / NP protein (His Tag)
Size: 100μg
Catalogue Number: PKSV030235-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Nucleoprotein; NP
Target Symptom: coronavirus NP Protein; MERS-CoV; coronavirus Nucleocapsid Protein; MERS-CoV; coronavirus Nucleoprotein Protein; MERS-CoV; cov np Protein; MERS-CoV; ncov NP Protein; MERS-CoV; novel coronavirus Nucleoprotein Protein; MERS-CoV; NP Protein; MERS-CoV; Nucleocapsid Protein; MERS-CoV; Nucleoprotein Protein; MERS-CoV
Target Species: MERS-CoV
Expression Host: Baculovirus-Insect Cells
Fusion Tag: C-His
Accession: AFS88943.1
Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Sequence: Met1-Asp413
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 46.51 kDa.
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only