Recombinant Mouse BACE2/Beta secretase 2 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSM040382-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: BACE2; Beta secretase 2
Target Symptom: 1110059C24Rik; AEPLC; AI850424; ALP56; ARP1; ASP21; BAE2; CDA13; CEAP1; DRAP
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-His
UNIProt ID: Q9JL18
Accession: Q9JL18
Background: BACE2, also known as beta secretase 2, belongs to the peptidase A1 family. It is a protease known to be an important enzyme involved in the cellular pathways. BACE2 has been shown to interact with GGA1 and GGA2. It is the major β -secretase in vivo. BACE2 is located on chromosome 21 and may play a role in alzheimer's disease pathogenesis in down syndrome(DS). Overexpression of BACE2 by lentivirus markedly reduced amyloid β protein production in primary neurons. Despite an extra copy of the BACE2 gene in DS and the increase of its transcription, BACE2 protein levels are unchanged.
Activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is > 50 pmoles/min/μg.
Sequence: Met1-Pro462
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 49.2 kDa
Observed MW: 55 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only