Recombinant Mouse Carbonic Anhydrase 4/CA4 Protein (aa 18-277, His Tag)
Sizes: 10μg, 50μg
Catalogue Numbers: PKSM040973-10, PKSM040973-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Carbonic Anhydrase 4; CA4
Target Synonym: CA4; CAIV; CA-IV; Car4; Carbonate dehydratase IV; carbonic anhydrase 4; carbonic anhydrase IVRP17; carbonic dehydratase IV; EC4.2.1.1; retinitis pigmentosa 17; RP17
UNIProt ID: Q64444
Target Species: Mouse
Expression Host: HEK293 Cells
Fusion Tag: C-6His
Accession: Q64444
Background: Carbonic anhydrase 4(CA4) is an enzyme that belongs to the alpha-carbonic anhydrase family. CA4 consists of a signal peptide (residues1-17), an ectodomain (residues18-277) and a propeptide (residues278-305), which is removed in the mature form. it is predominantly expressed in the embryo. CA4 can catalyzes the reversible reaction of CO2+H2O=HCO3-+H+, and stimulates the sodium/bicarbonate transporter activity of SLC4A4. Studies have shown that this protein have a role in inherited renal abnormalities of bicarbonate transport. Alpha-carbonic anhydrase family participate in avariety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor. They show extensive diversity in tissue is attribution and in their sub cellular localization.
Sequence: Glu18-Ser277
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 30.5 kDa
Observed MW: 36 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only