Recombinant Mouse Carbonic Anhydrase X/CA10 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSM040494-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Carbonic Anhydrase X; CA10
Target Symptom: 2700029L05Rik; BB085816; Ca10; Car10; RP23-448I9.1
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-His
UNIProt ID: P61215
Accession: P61215
Background: Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.
Activity: Measured by its esterase activity. The specific activity is > 10 pmoles/min/μg.
Sequence: Met 1-Asn 300
Purity: > 97 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 33.1 kDa
Observed MW: 38 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only