Recombinant Mouse Carboxypeptidase B1/CPB1 Protein (His Tag)(Active)
Size: 50μg
Catalogue Number: PKSM040708-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: Carboxypeptidase B1; CPB1
Target Symptom: 0910001A18Rik; 1810063F02Rik; 2210008M23Rik; AI504870
Target Species: Mouse
Expression Host: HEK293 Cells
Application: Enzyme
Fusion Tag: C-His
Accession: NP_083982.1
Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Activity: Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is > 5, 000 pmoles/min/μg.
Sequence: Met 1-Tyr 415
Purity: > 94 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 47.5 kDa
Observed MW: 43-45 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only