Recombinant Mouse CD86/B7-2 Protein (His Tag)(Active)
Size: 100μg
Catalogue Number: PKSM040944-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: CD86; B7-2
Target Symptom: T-lymphocyte activation antigen CD86; Activation B7-2 antigen; Early T-cell costimulatory molecule 1; ETC-1; CD86; B7; B7-2; B7.2; B70; Cd28l2; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2
Target Species: Mouse
Expression Host: HEK293 Cells
Application: ELISA
Fusion Tag: C-His
Accession: NP_062261.3
Background: CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
Activity: Immobilized mouse CD86 at 20 μg/ml (100 μl/well) can bind mouse CD28 with a linear ranger of 6. 4-800 ng/ml.
Sequence: Met 1-Glu 245
Purity: > 92 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 26.8 kDa
Observed MW: 40-55 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only