Recombinant Mouse CD8a Protein
Sizes: 10ug, 20g, 50ug, 100ug
Catalogue Numbers: RP01513-10, RP01513-20, RP01513-50, RP01513-100
Citations, Manuals and MSDS Available upon request.
Description: Recombinant Mouse CD8a Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys28-Tyr196) of mouse CD8 alpha (Accession #NP_001074579.1) fused with a 6×His tag at the C-terminus.
Alternate Names: Ly-2, Ly-B, Ly-35, Lyt-2, CD8A
SWISS: P01731
GeneID: 12525
Species: Mouse
Purity: ≥ 95 % as determined by SDS-PAGE.
Storage: Store at -20 ℃. Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Background: T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Bio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse CD8A at 2μg/mL (100 μL/well) can bind anti Mouse CD8A antibody with a linear range of 0.001-3.77μg/mL.
Source: HEK293 cells
Tag: C-His
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Antigen Sequence: KPQAPELRIFPKKMDAELGQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSAMRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSPVHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIY
Endotoxin: < 0.1 EU/μg of the protein by LAL method.
Research Use Only