Recombinant Mouse DDR1 Kinase/MCK10 Protein (His &GST Tag)(Active)
Size: 50μg
Catalogue Number: PKSM040294-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: DDR1 Kinase; MCK10
Target Symptom: 6030432F18; AI323681; Cak; CD167a; Nep; PTK3A
Target Species: Mouse
Expression Host: Baculovirus-Insect Cells
Application: Enzyme
Fusion Tag: N-His-GST
UNIProt ID: Q03146
Accession: Q03146-2
Background: Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
Activity: The specific activity was determined to be 2 nmol/min/mg using synthetic modified AXLtide peptide (modified-CKKSRGDYMTMQIG) as substrate.
Sequence: Leu444-Val874
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Not Applicable
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Calculated MW: 75.8 kDa
Observed MW: 68 kDa
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Research Use Only